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Zytokinbestimmung aus Glaskörperproben bei retinalen Gefäßerkrankungen

Cytokine determination from vitreous samples in retinal vascular diseases

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Zusammenfassung

Ansatz

Es erfolgte eine Bestimmung von Zytokinen aus Glaskörperproben von zuvor unbehandelten Patienten mit diabetischer Retinopathie (DRP), retinalem Venenverschluss (RVV) und altersbedingter Makuladegeneration (AMD) mit subretinaler Blutung.

Methode

Bei 187 Patienten (Median 67,0 Jahre, 101 Männer) wurde eine Kombinationstherapie inklusive einer 23-Gauge-Kernvitrektomie durchgeführt. Interleukin-6 (IL-6) und „monocyte chemoattractant protein-1“ (MCP-1) und „vascular endothelial growth factor“ (VEGF) wurden mittels „cytometric bead array“ (CBA) bestimmt und mit Kontrollproben verglichen.

Ergebnisse

Alle Erkrankungen hatten signifikant erhöhte Zytokinspiegel gegenüber der Kontrollgruppe außer VEGF bei AMD. Bei der DRP zeigten sich höhere VEGF-A- und MCP-1-Spiegel beim diffus zystoiden Makulaödem verglichen mit fokal zystoidem Ödem. Die ischämische DRP hatte höhere VEGF-A-Spiegel als die nichtischämische. Alle gemessenen Zytokine waren bei Zentralvenenverschlüssen höher als bei Venenastverschlüssen.

Zusammenfassung

Unterschiedliche intravitreale Zytokinspiegel konnten zwischen DRP, RVV und feuchter AMD dargestellt werden. Die genauere Kenntnis der spezifischen Ausprägung der Zytokindysregulation könnte eine zielgerichtetere Therapie zukünftig ermöglichen.

Abstract

Purpose

The aim of this study was to determine cytokine levels from vitreous samples of treatment-naive patients with diabetic retinopathy (DRP), retinal vein occlusion (RVO) and exudative age-related macular degeneration (ARMD).

Methods

In this study 187 patients (median age 67 years, 101 males) were treated with a combined drug therapy including a 23-gauge core vitrectomy. Interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1) and intravitreal vascular endothelial growth factor (VEGF-A) levels were determined a using cytometric bead assay (CBA) and compared to those of the control group.

Results

Compared to the control group all diseases had significantly elevated cytokine levels, except VEGF in ARMD. In DRP samples of patients with diffuse diabetic macula edema (DME) higher VEGF-A and MCP-1 levels were found than in patients with focal DME. Ischemic DRP had higher VEGF levels than non-ischemic DRP. All measured cytokines were significantly higher in central retinal vein occlusion (CRVO) than in branch retinal vein occlusion (BRVO).

Conclusions

Differences in intravitreal cytokine levels in DRP, RVO and ARMD could be demonstrated. The knowledge of depicted specific characteristic dysregulation of cytokines could allow more targeted future therapies.

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Authors and Affiliations

  1. Universitätsaugenklinik, Goethe Universität, Theodor Stern Kai 7, 60590, Frankfurt am Main, Deutschland

    M. Pfister, F.H. Koch, P. Singh & M.J. Koss

  2. Institut für medizinische und experimentelle Virologie, Goethe Universität, Frankfurt am Main, Deutschland

    J. Cinatl & F. Rothweiler

  3. Zentrum für Kinder- und Jugendmedizin, Ambulanz für Pneumologie und Allergologie, Allergologisches Labor, Goethe Universität, Frankfurt am Main, Deutschland

    R. Schubert

  4. Institut für Biostatistik und mathematische Modellierung, Goethe Universität, Frankfurt am Main, Deutschland

    H. Ackermann

  5. Doheny Eye Institute, University of Southern California, Los Angeles, USA

    M.J. Koss

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  1. M. Pfister
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  2. F.H. Koch
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  3. J. Cinatl
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  4. F. Rothweiler
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  5. R. Schubert
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  6. P. Singh
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  7. H. Ackermann
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  8. M.J. Koss
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Correspondence to M. Pfister.

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Die vorliegende Arbeit wurde bereits auf dem WOC 2009 präsentiert.

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Pfister, M., Koch, F., Cinatl, J. et al. Zytokinbestimmung aus Glaskörperproben bei retinalen Gefäßerkrankungen. Ophthalmologe 110, 746–754 (2013). https://doi.org/10.1007/s00347-012-2719-4

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  • DOI: https://doi.org/10.1007/s00347-012-2719-4

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