Transglutaminase activity in pancreatic islets

https://doi.org/10.1016/0304-4165(83)90378-1Get rights and content

Abstract

1. Pancreatic islet homogenates catalyze, in a Ca2+-dependent fashion, the incorporation of [2,5-3H]histamine, [1,4-14C]putrescine, [1,2-3H]agmatine, [14C]methylamine, L-[U-14C]lysine in N,N-dimethylcasein. 2. Using [2,5-3H]histamine as the amine donor, the Km for Ca2+ and histamine amounts to 90μM and 0.7 mM, respectively. 3. The incorporation of [2,5-3H]histamine into N,N-dimethylcasein is inhibited by monodansylcadaverine, N-p-tosyl glycine, bacitracin and methylamine, the relative extent of inhibition depending on the respective concentrations of Ca2+, inhibitor and amine donor. 4. Bacitracin and methylamine, but not N-p-tosyl glycine, cause a dose-related inhibition of glucose-stimulated insulin release. 5. It is concluded that, in pancreatic islets, the Ca2+-responsive transglutaminase activity plays a critical role in the process of glucose-induced insulin release.

References (24)

  • B.G. Kasson et al.

    Biochim. Biophys. Acta

    (1981)
  • P. Lebrun et al.

    Biochim. Biophys. Res. Commun.

    (1982)
  • J. Schrode et al.

    J. Biol. Chem.

    (1978)
  • H.G. Williams-Ashman et al.

    Biochem. Biophys. Res. Commun.

    (1977)
  • W.J. Malaisse et al.

    Ann. N.Y. Acad. Sci.

    (1978)
  • C.B. Wollheim et al.

    Physiol. Rev.

    (1981)
  • B. Formby et al.

    Am. J. Physiol.

    (1976)
  • A. Owen et al.

    Enzyme

    (1983)
  • I. Atwater et al.

    J. Physiol.

    (1979)
  • J.C. Henquin

    Nature

    (1979)
  • I. Valverde et al.

    Science

    (1979)
  • I. Valverde et al.

    Endocrinology

    (1981)
  • Cited by (40)

    • Oxidative degradation of polyamines in rat pancreatic hypertrophy

      1998, Biochimica et Biophysica Acta - Molecular Basis of Disease
    View all citing articles on Scopus
    View full text